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Dr Peter H. Barry is
an Emeritus and Conjoint Professor in the School of Medical Sciences and Department of
Physiology & Pharmacology, being previously a Professor of Physiology in the School of
Physiology and Pharmacology, at the University of New South Wales in Sydney, Australia from
1994-2001 and previously also Sub-Dean (Information Technology) in the Faculty of
Medicine there from 1995-1998. He originally graduated from the University of Sydney
(Australia) with a BSc (Hons) in Physics in 1963, followed by a PhD in Biophysics in 1968.
He then went to the University of California at Los Angeles (UCLA) to work with Dr Jared
Diamond for 3 years, followed by just over a year at the Physiological Laboratory in the
University of Cambridge, working with Dr Richard Adrian. He then returned to Australia as
a Queen Elizabeth II Fellow, collaborating with Dr Peter Gage before taking up a
lectureship there in 1974. In 1991, he was awarded a DSc by the University of New South
Wales for his research in Membrane Biophysics. In 1999, he was awarded the
Vice-Chancellor's Award for Teaching Excellence Using Educational Technology. He
was the President of the Australian Society for Biophysics
in 2003 & 2004.
In 2006, he was awarded the Bob Robertson Medal for Biophysics. For other information, see also:
http://notes.med.unsw.edu.au/resinterests.nsf/sw/7201054 |
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He and his research group are
currently using the patch-clamp technique in their research in two main areas. In one,
supported by the National Health and Medical Research Council of Australia, they are
exploring the mechanism of ion permeation in GABA and glycine receptor channels in
cultured neurons and in point-mutated recombinant receptors in order to relate channel
properties to molecular structure. In the second, supported by the Australian Research
Council, they are investigating the role of ionic channels in olfactory transduction in
olfactory receptor neurons. In addition, he has for many years had an interest in the role
of unstirred-layers adjacent to membranes on membrane transport processes and in the
problems of liquid junction potential corrections in electrophysiological measurements. He
has also investigated the electrical circuit problems associated with patch-clamping cells
and particularly problems associated with patch clamping small cells.
Some of these research interests
and teaching experience led to his development of a series of interactive graphical
computer programs for electrophysiology, MEMPOT,
ARTMEM and MEMCABLE,
which are being marketed internationally. These programs, written in C/C++, were designed
to simulate actual physiological experiments and clarify electrophysiological concepts
that students had difficulties understanding. MEMPOT , ARTMEM
and MEMCABLE have now been translated by Mr Ted Crawford, in
conjunction with him, at the University of New South Wales into a full Windows’
version, MemPotW, ArtMemW and MemCableW.
Resulting from Professor Barry's
interest in liquid junction potentials over a couple of decades and many appeals for
consultation on the matter, particularly with their growing importance in current research
using the patch-clamp technique and bilayers, he then developed the JPCalc program, also in C/C++. The aims of
this program were to use graphical means to indicate where and how junction potentials
arise in a range of different circuit situations, to enable their calculation and then to
indicate clearly how the appropriate corrections need to be applied. JPCalc has now been translated by software
personnel at Axon Instruments Inc. (California) into a full Windows program,
originally as part of
their AxoScope package and more recently as
part of their pClamp package, with a version of the stand-alone program, JPCalcW, being distributed by Professor
Barry. For further information, e-mail him at P.Barry@unsw.edu.au.
For General information about available software, click
here.
List of Major Publications
Regular International Journal Papers
l.
Barry, P.H. & Hope, A.B. (1969). Electro-osmosis in membranes: effects of
unstirred layers and transport numbers: Part I: Theory. Biophys. J. 9 (5),
700-728.
2.
Barry, P.H. & Hope, A.B. (1969). Electro-osmosis in
membranes: effects of unstirred layers and transport numbers: Part II: Experimental. Biophys.
J. 9 (5), 729-757.
3.
Barry, P.H. & Hope, A.B. (1969). Electro-osmosis in Chara
and Nitella cells. Biochim. Biophys. Acta. 193, 124-128.
4.
Barry, P.H. & Diamond, J.M. (1970). Junction
potentials, electrode standard potentials, and other problems in interpreting electrical
properties of membranes. J. Membrane Biol. 3, 93-122.
5.
Barry, P.H. (1970). Volume flows and pressure changes
during an action potential in cells of Chara australis. I. Experimental results. J.
Membrane Biol. 3, 313-334.
6.
Barry, P.H. (1970). Volume flows and pressure changes
during an action potential in cells of Chara australis. II. theoretical
considerations. J. Membrane Biol. 3, 335-371.
7.
Barry, P.H. & Diamond, J.M. (1971). A theory of ion
permeation through membranes with fixed neutral sites. J. Membrane Biol. 4,
295-330.
8.
Wright, E.M., Barry, P.H. & Diamond, J.M. (1971).
The mechanism of cation permeation in rabbit gall-bladder: conductances, the
current-voltage relation, the concentration dependence of anion-cation discrimination, and
the calcium competition effect. J. Membrane Biol. 4, 331-357.
9.
Barry, P.H., Diamond, J.M. & Wright, E.M. (1971).
The mechanism of cation permeation on rabbit gall-bladder: dilution potentials and biionic
potentials. J. Membrane Biol. 4, 358-394.
10. Barry, P.H. & Adrian, R.H. (1973). Slow conductance
changes due to potassium depletion in the transverse tubules of frog muscle fibres during
hyperpolarizing pulses. J. Membrane Biol. 14, 243-292.
11. Barry, P.H. (1977). Transport number effects in the
transverse tubular system and their implications for low frequency impedance measurement
of capacitance of skeletal muscle fibers. J. Membrane Biol. 34, 383-408.
12. Barry, P.H., Gage, P.W. & Van Helden, D.F. (1979).
End-plate channels behave as neutral site channels. Neuroscience Letters 11,
233-237.
13. Barry, P.H., Gage, P.W. & Van Helden, D.F. (1979).
Cation permeation at the amphibian motor end-plate. J. Membrane Biol. 45,
245-276.
14. Barry, P.H., Gage, P.W. & Van Helden, D.F. (1979).
Cation permeation through single motor end-plate channels. Excerpta Medica,
International Congress Series, 473, 174-184.
15. Gage, P.W., Hamill, O.P., Van Helden, D.F. & Barry,
P.H. (1979). Acetylcholine receptors and end-plate channels. Excerpta Medica,
International Congress Series, 473, 166-173.
16. Takeda, K., Barry, P.H. & Gage, P.W. (1980).
Effects of ammonium ions on end-plate channels. J. Gen. Physiol., 75,
589-613.
17. Takeda, K., Gage, P.W. & Barry, P.H. (1982).
Effects of divalent cations on toad end-plate channels. J. Membrane Biol., 64,
55-66
18. Takeda, K., Barry, P.H. & Gage, P.W. (1982).
Effects of extracellular sodium concentration on null potential, conductance and open time
of end-plate channels. Proc. Roy. Soc. (London) B. 216, 225-251.
19. Barry, P.H. & Dulhunty, A.F. (1984). Slow potential
changes in mammalian muscle fibers during prolonged hyperpolarization: transport number
effects and chloride depletion. J. Membrane Biol., 78: 235-248.
20. Barry, P.H. (1984). Slow potential changes due to
transport number effects in cells with membrane invaginations or dendrites. J. Membrane
Biol., 82: 221-239.
21. Quartararo, N., Barry, P.H. and Gage, P.W. (1987). Ion
permeation through single channels activated by acetylcholine in denervated toad sartorius
skeletal muscle fibres: Effects of alkali cations. J. Membrane Biol., 97:
137-159.
22. Quartararo, N. & Barry, P.H. (1987). A simple
technique for transferring excised patches of membrane to different solutions for single
channel measurements. Pflügers Archiv 410: 677-678.
23. Quartararo, N. & Barry, P.H. (1988). Ion permeation
through single ACh-activated channels in denervated toad sartorius skeletal muscle fibres:
effect of temperature. Pflügers Archiv 411: 101-112.
24. Lynch, J.W, Barry, P.H. & Quartararo, N. (1988). A
temperature and solution control system for the measurement of single channel currents in
excised membrane patches. Pflügers Archiv 412: 322-327.
25. Lynch, J.W. and Barry, P.H. (1989). Action potentials
initiated by single channels opening in a small neuron (rat olfactory receptor). Biophys.
J., 55: 755-768.
26. *Barry, P.H. and Quartararo, N. (1990). PNSCROLL, a
software package for graphical interactive analysis of single channel patch clamp currents
and other binary file records: under mouse control. Comput. Biol. Med. 20:
193-204.
27. Barry, P.H. (1990). A membrane potential simulation
program for IBM-PC-Compatible equipment for students in the physiological and biological
sciences. Am. J. Physiol (259, Adv. Physiol.Educ. 4), S15-S23.
28. Lynch, J.W. and Barry, P.H. (1991). Properties of
transient K+ currents and underlying single K+ channels in rat olfactory receptor neurons.
J. Gen. Physiol. 97: 1043-1072.
29. Lynch, J.W. and Barry, P.H. (1991). Inward
rectification in rat olfactory receptor neurones. Proc. Roy. Soc. (Series B) 243:
149-153.
30. Lynch, J.W. and Barry, P.H. (1991). Slowly-activating
K+ channels in rat olfactory receptor neurons. Proc. Roy. Soc. (Series B) 244:
219-225.
31. Rajendra, S., Lynch J.W. and Barry, P.H (1992). An
analysis of Na+ currents in rat olfactory receptor neurons. Pflügers Archiv. 420:
342-346.
32. Vandenberg, R.J., French, C.R., Barry, P.H., Shine, J.
and Schofield, P. (1992). Competitive antagonism of ligand-gated ion channel receptors:
two domains of the glycine receptor a subunit form the strychnine binding site. Proc.Nat.Acad.Sci.USA
89: 1765-1769.
33. Fatima-Shad, K. and Barry, P.H. (1992). A patch-clamp
study of GABA- and strychnine-sensitive glycine-activated currents in post-natal
tissue-cultured hippocampal neurons. Proc. R. Soc. Lond. B. 250: 99-105.
34. Fatima-Shad, K. and Barry, P.H. (1993). Anion
permeation in GABA- and glycine-gated channels in mammalian cultured hippocampal neurons. Proc.
R. Soc. Lond. B. 253: 69-75.
35. Vandenberg, R.J., Rajendra, S.R., French, C.R., Barry,
P.H. and Schofield, P. (1993). The extracellular disulfide loop motif of the inhibitory
glycine receptor does not form the agonist binding site. Mol. Pharm., 44:
198-203.
36. Barry, P.H. (1994) JPCalc - a software package
for calculating liquid junction potential corrections in patch-clamp, intracellular,
epithelial and bilayer measurements and for correcting junction potential measurements.
J. Neurosci. Methods, 51: 107-116.
37. Barry, P.H. (1994) ARTMEM - An interactive
graphical program simulating membrane potential measurements across artificial membranes. Ann.
Biomed. Eng., 22: 218-225.
38. Rajendra, S., Lynch, J.W., Pierce, K., French, C.R.,
Barry, P.H. and Schofield, P. (1994). Startle disease mutations reduce the agonist
sensitivity of the human inhibitory glycine receptor. J. Biol. Chem. 269:
18739-18742.
39. Ng, B. and Barry, P.H. (1995). The measurement of ionic
conductivities and mobilities of certain less common organic ions needed for junction
potential corrections in electrophysiology. J. Neurosci. Methods, 56: 37-41
40. Rajendra, S., Lynch, J.W., Pierce, K., French, C.R.,
Barry, P.H. and Schofield, P.R. (1995) Mutation of an arginine residue in the human
glycine receptor transforms b -alanine and taurine from agonists into competitive
antagonists. Neuron, 14: 169-175.
41. Rajendra, S., Vandenberg, R.J., Pierce, K., Cunningham,
A.M., French, P.W., Barry, P.H. and Schofield, P.R. (1995) The unique extracellular
disulfide loop of the glycine receptor is a principal ligand binding element. EMBO J., 14:
2987-2998.
42. Lynch, J.W., Rajendra, S., Barry, P.H. and Schofield,
P.R. (1995) Mutations affecting the glycine receptor agonist transduction mechanism
convert the competitive antagonist, picrotoxin, into an allosteric potentiator. J.
Biol. Chem. 270: 13799-13806.
43. Balasubramanian, S., Lynch, J.W. and Barry, P.H. (1995)
The permeation of organic cations through cAMP-gated channels in mammalian olfactory
receptor neurons. J. Membrane Biol. 146: 177-191.
44. Fatima-Shad, K. and Barry, P.H. (1995). Heterogeneous
current responses to GABA and glycine are present in post-natally cultured hippocampal
neurons. Brain Res. 704: 246-255.
45. Balasubramanian, S., Lynch, J.W. and Barry, P.H.
(1996). Calcium-dependent modulation of the agonist affinity of the mammalian olfactory
cyclic nucleotide-gated channel by calmodulin and a novel endogenous factor. J.
Membrane Biol. 152: 13-23.
46. Schofield, P.R., Lynch, J.W., Rajendra, S., Pierce,
K.D., Handford, C.A. and Barry, P.H. (1996). Molecular and genetic insights into ligand
binding and signal transduction at the inhibitory glycine receptor. Cold Spring Harbor
Symposium on Quantitative Biology 61: 333-342
47. Chiu, P., Lynch, J. W. and Barry, P. H. (1997).
Odorant-induced currents in intact patches from rat olfactory receptor neurons: theory and
experiment. Biophys. J., 72: 1442-1457.
48. Lynch, J.W., Rajendra, S., Pierce, K.D., Handford,
C.A., Barry, P.H. and Schofield, P.R. (1997). Identification of intracellular and
extracellular domains mediating signal transduction in the inhibitory glycine receptor
chloride channel. EMBO J. 16: 110-120.
49. Balasubramanian, S., Lynch, J.W. and Barry, P.H.
(1997). Concentration dependence of cation permeation and cation interactions in the
cyclic AMP-gated channels of mammalian olfactory receptor neurons. J. Membrane Biol.
159: 41-52.
50. Hallani, M., Lynch, J.W. and Barry, P.H. (1998).
Characterisation of calcium-activated chloride channels in patches excised from the
dendritic knob of olfactory receptor neurons. J. Membrane Biol. 161: 163-171.
51. Fatima-Shad, K. and Barry, P.H. (1998). Morphological
and electrical characteristics of post-natal hippocampal neurons in culture: the presence
of bicuculline- and strychnine-sensitive IPSPs. Tissue & Cell. 30:
236-250.
52. Barry, P.H. (1998). The derivation of unstirred-layer
transport number equations from the Nernst-Planck Equations. Biophys. J., 74:
2903-2905.
53. Moorhouse, A.J., Jacques, P., Barry, P.H. and
Schofield, P.R. (1999). The startle disease mutation Q266H, in the second transmembrane
domain of the human glycine receptor, impairs channel gating. Mol. Pharm., 55:
386-395.
54. Keramidas, A., Kuhlmann, L., Moorhouse, A.J. and Barry,
P.H. (1999). Measurement of the limiting equivalent conductivities and mobilities of the
most prevalent ionic species of EGTA (EGTA2- and EGTA3-) for use in
electrophysiological experiments. J. Neurosci. Meth. 89: 41-47
55. Qu, W., Moorhouse, A.J., Rajendra, S. and Barry, P.H.
(2000). Very negative potential for half-inactivation of, and effects of anions
on, voltage-dependent sodium currents in acutely isolated rat olfactory receptor neurons. J.
Membrane Biol., 175: 175: 123-138.
56. Keramidas, A., Moorhouse, A.J., French, C.R.,
Schofield, P.R. and Barry, P.H. (2000). M2 pore mutations convert the glycine receptor
channel from being anion to cation selective. Biophys. J., 78: 247-259.
57. Qu, W., Zhu, X. O., Moorhouse, A.J., Bieri, S.,
Cunningham, A.M. and Barry, P.H. (2000). Ion Permeation and Selectivity of
Wild-Type Recombinant Rat CNG (rOCNC1) Channels Expressed in HEK293 Cells. J. Membrane
Biol., 178: 137-150.
58. Friedrich, O. Ehmer, T., Uttenweiler, D., Vogel, M.,
Barry, P.H. and Fink, R.H.A. (2001) Numerical Analysis of Ca2+ depletion in the
transverse tubular system (TTS) of mammalian muscle. Biophys. J., 80: 2046-2055.
59. Kaur, R., Zhu, X.O., Moorhouse, A.J. and Barry,
P.H. (2001). IP3-gated channels and their occurrence relative to CNG channels
in the soma and dendritic knob of rat olfactory receptor neurons.
J. Membrane
Biol, 181: 181: 91-105.
60. Qu, W., Moorhouse, A. J., Cunningham, A.M. and P. H.
Barry. (2001) Anomalous mole-fraction effects in recombinant and native cyclic
nucleotide-gated channels in rat olfactory receptor neurons. Proc. Roy. Soc. Lond. Ser.
B. 268: 1395-1403.
61. Zhu,
X.O., Qu, W., Moorhouse, A.J., Cunningham,
A.M. & Barry, P.H. (2001) Cyclic Nucleotide activation and spermine block of
recombinant a-homomeric
olfactory rat cyclic nucleotide-gated channels. Sensory Neuron, (in
press).
62. Keramidas, A., Moorhouse, A.J., Pierce,
K., Schofield, P.R. and Barry, P.H. (2002). Cation-selective mutations in the M2 domain of
the inhibitory glycine receptor channel reveal determinants of ion-charge selectivity.
J.
Gen. Physiol. 119: 393-410
63. Moorhouse, A.J., Keramidas, A.,
Zaykin, A., Schofield, P.R. and Barry, P.H. (2002). Single channel analysis of conductance
and rectification in cation-selective, mutant glycine receptor-channels.
J.
Gen. Physiol. 119: 411-425
64. O’Mara, M., Barry, P.H. and
Chung, S-H. (2003). A model of the glycine receptor deduced from Brownian
dynamics studies. P.N.A.S. 100: 4310-4315.
65. Barry, P.H. (2003).
Commentary. The relative contributions of cAMP and InsP3 pathways to
olfactory responses in vertebrate olfactory receptor neurons and the specificity
of odorants for both pathways. J. Gen. Physiol. 122: 247-250.
66. Lee,
D J-S, Keramidas,
A.,
Moorhouse,
A.J., Schofield, P.R. and Barry,
P.H. (2003).
The contribution of proline 250 (P-2’)
to pore diameter and ion selectivity in the human glycine receptor channel.
Neurosci. Letts. 351(3):
196-200.
67. Carland, J.E.,
Moorhouse, A.J., Barry, P.H., Johnston, G.A.R. and Chebib, M. (2004). Charged
residues at the 2' position of Human GABAC
r1
receptors invert ion selectivity and influence open state probability. J.
Biol. Chem. 279: 54153-54160.
68. Qu
W., Moorhouse, A. J., Lewis, T. M., Pierce, K. D. and Barry, P. H.
(2005). Mutation of the pore glutamate affects both cytoplasmic and external
dequalinium block in the rat olfactory CNGA2 channel. Eur. Biophys. J.
34: 442-453.
69.
Qu, W., Moorhouse, A. J., Chandra, M., Lewis, T. M., Pierce, K. D.
and Barry, P. H. (2006). A single P-loop glutamate point mutation to
either lysine or arginine switches the cation-anion selectivity of the CNGA2
channel. J. Gen. Physiol.
127: 375-389.
70.
Barry, P.H. (2006). The reliability of relative anion-cation permeabilities
deduced from reversal (dilution) potential measurements in ion channel studies.
Cell Biochem. Biophys. 46: 143-154.
71.
Sugiharto, S., T. M. Lewis, A. J. Moorhouse, P. R. Schofield, and
P. H. Barry. 2008. Anion-cation permeability correlates with hydrated
counter-ion size in glycine receptor channels. Biophys. J. 95:
4698-4715.
B. Invited
Refereed Reviews
72. Barry, P.H. (1974). Cation permeability and selectivity
in biological membranes. Proc. Aust. Physiol. Pharmacol. Soc. 5, 10-17.
73. Barry, P.H. (1983). The effects of unstirred-layers on
the movement of ions across cell membranes. Review in: Proc. Aust. Physiol. Pharmacol.
Soc., 14: 152-169.
74. Barry, P.H., & Diamond, J.M. (1984). Effects of
unstirred layers on membrane phenomena.
Physiol. Rev., 64: 763-872.
75. Barry, P.H. and Lynch, J.W. (1991). Topical Review.
Liquid junction potentials and small cell effects in patch clamp analysis. J. Membrane
Biol. 121: 101-117.
76. Lynch, J.W. and Barry, P.H. (1992). Studying olfactory
transduction using patch-clamping. Today's Life Science, 4: 26-42.
77. Barry, P.H. and Fatima-Shad, K. (1995). Amino acid
residues and control of ion permeation through a ligand-gated ion channel superfamily. Today's
Life Science, 7: 32-37.
78. Barry, P.H., Schofield, P.R. and Moorhouse, A.J.
(1999). Glycine receptors: What gets in and why? Clin. Exp. Pharm. Phys. 26:
935-936.
79. Keramidas, A., Moorhouse, A.J.,
Schofield, P.R. and Barry, P.H. (2004). Ligand gated ion channels: Mechanisms
underlying ion selectivity. Invited review for:
Prog. Biophys. Molec.
Biol., 86(2): 161-204.
80.
Barry, P.H. and Lynch, J.W. (2005).
Ligand-gated channels. IEEE Trans. Nanobiosci. 4 (1) March:
70-80.
Invited Chapters in Books
81. Diamond, J.M., Barry, P.H. & Wright, E.M. (1971).
The route of trans- epithelial ion permeation in the gall-bladder. From: Electrophysiology
of Epithelial Cells, pp 23-33. Symposium chairman: G. Giebisch. F.K. Schattauer
Verlag. Stuttgart-New York.
82. Dulhunty, A.F., Gage, P.W. & Barry, P.H. (1981).
Asymmetrical charge movement in normal and glycerol-treated toad sartorius fibres. Adv.
Physiol. Sci. Vol. 5. In: Molecular and Cellular Aspects of Muscle Function.
E. Varga, A. Kover, T. Kovacs (eds), 321-327.
83. Barry, P.H. (1981). Unstirred layers and volume flows
across biological membranes. In: Water Transport across Epithelia. Alfred
Benzon Symposium 15, 132-146 (and 147-153 of discussion).
84. Barry, P.H. & Gage, P.W. (1984). Ion selectivity of
channels at the end-plate. In: Current Topics in Membranes and Transport, (Academic
press), 21: 1-51.
85. Barry, P.H. (1989). Permeation mechanisms in epithelia:
Biionic potentials, dilution potentials, conductances and streaming potentials. In: Methods
in Enzymology, Biomembranes, Part M: Biological Transport, 171: 678-715.
86. Barry, P.H. and McLachlan, E. (1990). Electrochemistry
of membranes and excitable cells. In: Electropharmacology, CRC Press (Eds. H.
Keyzer, G.M. Eckert and F. Gutmann), pp. 107-138.
87. Barry, P.H. (1992). Graphical simulation of
experimental measurements of membrane potentials. In: Methods in Neurosciences, 10
(Ed. P.M. Conn, Academic Press), 473-491.
88. Barry, P.H., Lynch, J.W. & Rajendra, S. (1994).
Patch-clamp measurements on mammalian olfactory receptor neurons. In: Olfaction and
Taste XI, (Eds. K. Kurihara & N. Suzuki; Springer Verlag), pp. 168-172.
89. Barry, P.H., Chiu, P. & Lynch, J.W. (1998). An
analysis of odorant-induced currents in on-cell patches on mammalian olfactory receptor
neurons. In: Olfaction and Taste XII, (Ed. Claire Murphy), Annals New York Acad.
Sci., 855, 208-211.
90. Barry, P.H., Balasubramanian, S. & Lynch, J.W.
(1999). How Sensory cells encode information: the processes that underlie sensitivity to
chemical stimuli, their quality and their quantity. In: Tastes and Aromas. The Chemical
Senses in Science and Industry (Eds. G.A. Bell and A.J. Watson, UNSW Press: Sydney),
pp. 120-129.
91. Lynch, J.W. & Barry, P.H.
(2007).
Ligand-gated channels: permeation and activation. In:
Biological Membrane Ion Channels: Dynamics, Structure,
and Applications. Chung, S. H., Krishnamurthy, V.
and Andersen, O. S. (eds.), Springer, New York, pp.
334-367.
92. Barry, P.H., Qu, W. & Moorhouse, A.J. (2008).
Biophysics of CNG ion channels. In: Sensing with Ion Channels. (Ed.
B. Martinac), Springer-Verlag, Heidelberg, pp. 175-200.
Plus a number of separately authored
mathematical appendices, full conference papers and a large number of conference
abstracts.
For General information about available software,
click here.